| Final | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Islay-Mae Underwood | 12 | Kettering | 40.31 |
| 2 | 2 | Beth Rebhan | 12 | Corby | 39.64 |
| 3 | 3 | Kimberley Hayes | 12 | Kettering | 39.04 |
| 4 | 4 | Sofia Reedha | 12 | Corby | 34.95 |
| 5 | 5 | Teagan Payne | 12 | Northampton | 37.93 |
| 6 | 6 | Isobelle Frow | 12 | Corby | 39.22 |
| 7 | 7 | Holly Coleman | 12 | Wellingboro | 39.87 |
| 8 | 8 | Lexie Webb | 12 | Rushden | 42.52 |