| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 2 | 1 | Sui Chun Yip | 11 | Wellingboro | 53.32 |
| 3 | 5 | Jack Tindle | 11 | Northampton | 44.12 |
| 4 | 9 | Kushal Mistry | 12 | Northampton | 41.46 |
| 5 | 7 | James Oscroft | 10 | Northampton | 43.49 |
| 6 | 3 | Rahul Reedha | 10 | Corby | 48.86 |
| Heat Number - 2 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 2 | 2 | Grayson Watford | 10 | Rushden | 51.67 |
| 3 | 6 | Ryan Doyle | 12 | Kettering | 43.61 |
| 4 | 10 | Jakub Siedlak | 12 | Corby | 41.04 |
| 5 | 8 | Jack Webb | 12 | Wellingboro | 43.10 |
| 6 | 4 | Oscar Venn | 11 | Northampton | 45.92 |