| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Olivia FAUSTMANN | 12 | Leic Sharks | 3:13.97 |
| 2 | 3 | Eleanor TRAHEARN | 14 | Hinckley | 2:55.46 |
| 3 | 6 | Matilda HERRIES | 15 | Loughborough | 2:39.45 |
| 4 | 8 | Francesca PALFREY | 17 | Loughborough | 2:29.30 |
| 5 | 7 | Elizabeth PALFREY | 15 | Loughborough | 2:35.15 |
| 6 | 4 | Abigail GOLYNKER | 13 | Leic Sharks | 2:55.11 |
| 7 | 2 | Chloe GENT | 13 | Coalville | 2:55.98 |